Standardized methods to rear high‐quality <i>Galleria mellonella</i> larvae for the study of fungal pathogens

The greater wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae), has gathered widespread interest in the biomedical research community as an alternative non-mammalian model to study bacterial and fungal infections humans. Nevertheless, most laboratories rely on commercial breeding companies that grow these larvae bulk. Variation rearing conditions between shipping of affects response G. infection, both decreasing reproducibility comparability infection experiments across laboratories. To unlock full potential model, standardized is essential produce healthy uniform quality. this end, we developed optimized protocol using a high-nutritional artificial diet cost-effective housing solutions ensure optimal during each stage life cycle. This resulted rapid generation with short larval phase, large size, increased weight compared other protocols larvae. A survival analysis comparing commercially obtained showed significantly higher reared after Candida albicans (CP Robin) Berkhout (Saccharomycetales), proving quality